Research papers

2022-

Chou TH, Kang H, Simorowski N, Traynelis S, Furukawa H*. Structural insights into assembly and function of GluN1-2C, GluN1-2A-2C, and GluN1-2D NMDARs (2022) Mol Cell. 2022 Online ahead of print.

Yovanno R, Chou TH, Furukawa H, Lau AY*. Excitatory and inhibitory D-serine binding to the NMDA receptor (2022) Elife. 2022 Oct 27;11:e77645. doi: 10.7554/eLife.77645. PMID: 36301074

Steigerwald R, Chou TH, Furukawa H, Wünsch B*. GluN2A-Selective NMDA Receptor Antagonists: Mimicking the U-Shaped Bioactive Conformation of TCN-201 by a [2.2]Paracyclophane System. (2022) ChemMedChem. 2022 Sep 28:e202200484. doi: 10.1002/cmdc.202200484. Online ahead of print. PMID: 36169098

Chou TH#, Epstein M#, Michalski K#, Fine E, Biggin PC, Furukawa H*. Structural insights into binding of therapeutic channel blockers in NMDA receptors. (2022) Nat Struct Mol Biol. 2022 May 30. doi: 10.1038/s41594-022-00772-0

Tajima N#, Simorowski N#, Yovanno RA, Regan MC, Michalski K, Gómez R, Lau AY*, Furukawa H*. Development and characterization of functional antibodies targeting NMDA receptors. (2022) Nat Commun. 2022 Feb 17;13(1):923. doi: 10.1038/s41467-022-28559-3.

2018-2021

Hansen KB, Wollmuth LP, Bowie D, Furukawa H, Menniti FS, Sobolevsky AI, Swanson GT, Swanger SA, Greger IH, Nakagawa T, McBain CJ, Jayaraman V, Low CM, Dell’Acqua ML, Diamond JS, Camp CR, Perszyk RE, Yuan H, Traynelis SF. (2021) Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels. (2021) Pharmacol Rev. 2021 Oct;73(4):298-487. doi: 10.1124/pharmrev.120.000131.

Syrjanen, J#, Michalski K#., Kawate, T. & Furukawa, H*. On the molecular nature of large-pore channels. (2021) Journal of Molecular Biology. 2021 Aug 20;433(17):166994. doi: 10.1016/j.jmb.2021.166994. Epub 2021 Apr 16.

Furukawa, H*., Simorowski, N. & Michalski, K. Effective production of oligomeric membrane proteins by EarlyBac-insect cell system. (2021) Methods Enzymol. 2021; 653:3-19. doi: 10.1016/bs.mie.2020.12.019. Epub 2021 Jan 18.

Chou TH#, Tajima N#, Romero-Hernandez A, Furukawa H* (2020) Structural Basis of Functional Transitions in Mammalian NMDA Receptors.   Cell 2020 Jun 26:S0092-8674(20)30685-1. doi: 10.1016/j.cell.2020.05.052. Online ahead of print. PMID: 32610085

Perszyk RE, Myers SJ, Yuan H, Gibb AJ, Furukawa H, Sobolevsky AI, Traynelis SF. (2020) Hodgkin-Huxley-Katz Prize Lecture: Genetic and pharmacological control of glutamate receptor channel through a highly conserved gating motif. J Physiol. 2020 May 29. doi: 10.1113/JP278086. Online ahead of print. PMID: 32468591

Michalski K#, Syrjanen JL#, Henze E, Kumpf J, Furukawa H*, Kawate T* (2020) The cryo-EM structure of a pannexin 1 reveals unique motifs for ion selection and inhibition. eLife 2020;9:e54670 doi: 10.7554/eLife.546

Syrjanen JL, Michalski K, Chou TH, Grant T, Rao S, Simorowski N, Tucker SJ, Grigorieff N, Furukawa H*. (2020) Structure and assembly of calcium homeostasis modulator proteins. Nat Struct Mol Biol. 2020 Jan 27 (2):150-159. doi: 10.1038/s41594-019-0369-9.

Wang JX, Irvine MW, Burnell ES, Sapkota K, Thatcher RJ, Li M, Simorowski N, Volianskis A, Collingridge GL, Monaghan DT, Jane DE*, Furukawa H*. (2020) Structural basis of subtype-selective competitive antagonism for GluN2C/2D-containing NMDA receptors. Nature Commun. 2020 Jan 22;11(1):423. doi: 10.1038/s41467-020-14321-0.

Regan MC, Zhu Z, Yuan H, Myers SJ, Menaldino DS, Tahirovic YA, Liotta DC, Traynelis SF, Furukawa H*. (2019) Structural elements of a pH-sensitive inhibitor binding site in NMDA receptors.  Nature Commun. 2019 Jan 18;10(1):321.

Wang JX, Furukawa H* (2019) Dissecting diverse functions of NMDA receptors by structural biology. Curr Opin Struct Biol. 2019 Jan 28;54:34-42

Hansen KB, Yi F, Perszyk RE, Furukawa H, Wollmuth LP, Gibb AJ, Traynelis SF. (2018) Structure, function, and allosteric modulation of NMDA receptors. J Gen Physiol. 2018 Aug 6;150(8):1081-1105.

Wu Q, Regan M, Furukawa H, Jackson D.  (2018) Role of heterotrimeric Gα proteins in maize development and enhancement of agronomic traits. PLoS Genet. Apr 30;14(4):e1007374.

Regan MC, Grant T, McDaniel MJ, Karakas E, Zhang J, Traynelis SF, Grigorieff N, Furukawa H*. (2018) Structural Mechanism of Functional Modulation by Gene Splicing in NMDA Receptors. Neuron. May 2;98(3):521-529.e3.

2014-2017

Elegheert J, Cvetkovska V, Clayton AJ, Heroven C, Vennekens KM, Smukowski SN, Regan MC, Jia W, Smith AC, Furukawa H, Savas JN, de Wit J, Begbie J, Craig AM, Aricescu AR. (2017) Structural Mechanism for Modulation of Synaptic Neuroligin-Neurexin Signaling by MDGA Proteins. Neuron. 2017 Aug 16;95(4):896-913.e10.

Amin JB, Salussolia CL, Chan K, Regan MC, Dai J, Zhou HX, Furukawa H, Bowen ME, Wollmuth LP. (2017) Divergent roles of a peripheral transmembrane segment in AMPA and NMDA receptors. J Gen Physiol. 2017 Jun 5;149(6):661-680.

Romero-Hernandez A, Furukawa H*. (2017) Novel mode of antagonist binding in NMDA receptors revealed by the crystal structure of the GluN1-GluN2A ligand-binding domain complexed to NVP-AAM077. Mol Pharmacol. Jul;92(1):22-29.

Romero-Hernandez A, Simorowski N, Karakas E, Furukawa H* (2016) Molecular Basis for Subtype Specificity and High-Affinity Zinc Inhibition in the GluN1-GluN2A NMDA Receptor Amino-Terminal Domain. Neuron. 2016 Dec 21;92(6):1324-1336.

Tajima N, Karakas E, Grant T, Simorowski N, Diaz-Avalos R, Grigorieff N*, Furukawa H* (2016) Activation of NMDA receptors and the mechanism of inhibition by ifenprodil. Nature. 2016 May 2;534(7605):63-8.

Regan MC, Romero-Hernandez A, Furukawa H*. (2015) NMDA receptor pharmacology and function. Curr Opin Struct Biol. 2015 Aug 14;33:68-75.

Karakas E, Regan MC, and Furukawa H* (2015).Emerging structural insights into the function of ionotropic glutamate receptors. Trends Biochem Sci. 2015 Jun;40(6):328-37.

Khatri A, Burger PB, Swanger SA, Hansen KB, Zimmerman S, Karakas E, Liotta DC, Furukawa H, Snyder JP, Traynelis SF. (2014). Structural determinants and mechanism of action of a GluN2C-selective NMDA receptor positive allosteric modulator. Mol Pharmacol. 86(5):548-60.

Karakas E, Furukawa H*. (2014) Crystal structure of a heterotetrameric NMDA receptor ion channel. Science, 344(6187): 992-997.

Jespersen A#, Tajima N#, Fernandez-Cuervo G, Garnier-Amblard E, Furukawa H* (2014). Structural insights into competitive antagonism in NMDA receptors. Neuron, 81(2):366-378.

2009-2013

Salussolia CL, Gan Q, Kazi R, Singh P, Allopenna J, Furukawa H, Wollmuth LP., (2013). A eukaryotic specific transmembrane segment is required for tetramerization in AMPA receptors. J Neurosci. 33(23): 9840-5.

Hansen KB, Tajima N, Risgaard R, Perszyk RE, Jørgensen L, Vance KM, Ogden KK, Clausen RP, Furukawa H*, Traynelis SF*. (2013). Structural determinants of agonist efficacy at the glutamate binding site of N-methyl-D-aspartate receptors. Mol Pharmacol. 84(1): 114-27.

Burger PB, Yuan H, Karakas E, Geballe M, Furukawa H, Liotta DC, Snyder JP, Traynelis SF., (2012). Mapping the Binding of GluN2B-Selective N-Methyl-D-aspartate Receptor Negative Allosteric Modulators. Mol Pharmacol, 82(2):344-359

Furukawa H*, (2012). Structure and function of glutamate receptor amino terminal domains. J. Physiol, 590(Pt 1): 63-72.

Karakas E, Simorowski N, and Furukawa H*, (2011). Subunit arrangement and phenylethanolamine binding in GluN1/GluN2B NMDA receptors. Nature, 475(7355): 249-253.

Vance K, Simorowski N, Traynelis S, Furukawa H*, (2011). Ligand-specific deactivation time course of GluN1/GluN2D NMDA receptors. Nature Commun, 2: 294.

Hansen K, Furukawa H, Traynelis, S, (2010). Control of assembly and function of glutamate receptors by the amino-terminal domain. Mol Pharmacol, 78: 535-549.

Karakas E, Simorowski N, Furukawa H*, (2009). Structure of the zinc-bound amino-terminal domain of the NMDA receptor NR2B subunit. EMBO J, 28(24): 3910-3920.

Previews

Regan MC, Furukawa H* (2016) Deeper Insights into the Allosteric Modulation of Ionotropic Glutamate Receptors. Neuron. 2016 Sep 21;91(6):1187-9.

Malinauskas T, Furukawa H* (2016) Production of Heteromeric Transmembrane Receptors with Defined Subunit Stoichiometry. Structure. 2016 May 3;24(5):653-5.

Once upon a time

Jin R, Singh SK, Gu S, Furukawa H, Sobolevsky AI, Zhou J, Jin Y, Gouaux E, (2009). Crystal structure and association behaviour of the GluR2 amino-terminal domain. EMBO J, 28(12):1812-1823.

Jasti J, Furukawa H, Gonzales E, Gouaux E, (2007). Structure of acid-sensing ion channel 1 at 1.9 Å resolution and low pH. Nature, 449(7160): 316-323.

Vásquez V, Cortes DM, Furukawa H, Perozo E, (2007). An optimized purification and reconstitution method for the MscS channel: strategies for spectroscopical analysis. Biochemistry, 46(23): 6766-6773.

Takahashi H, Shin Y, Cho SJ, Zago WM, Nakamura T, Gu Z, Ma Y, Furukawa H, Liddington R, Zhang D, Tong G, Chen HS, Lipton SA (2007). Hypoxia enhances S-Nitrosylation-Mediated NMDA receptor inhibition via a thiol oxygen sensor motif. Neuron, 53(1): 53-64.

Furukawa H, Singh SK, Mancusso R, Gouaux E, (2005). Subunit arrangement and function in NMDA receptors. Nature, 438(7065): 185-192.

Inanobe A, Furukawa H, Gouaux E, (2005). Mechanism of partial agonist action at the NR1 subunit of NMDA receptors. Neuron, 47(1): 71-84.

Furukawa H, Gouaux E., (2003). Mechanisms of activation, inhibition and specificity: crystal structures of the NMDA receptor NR1 ligand-binding core. EMBO J, 22(12): 2873-2885.

Furukawa H, Hamada T, Hayashi MK, Haga T, Muto Y, Hirota H, Yokoyama S, Nagasawa K, Ishiguro M, (2002). Conformation of ligands bound to the muscarinic acetylcholine receptor. Mol Pharmacol, 62(4): 778-787.

Furukawa H, Haga T, (2000). Expression of Functional M2 Muscarinic Acetylcholine Receptor in Escherichia coli. J Biochem, 127(1): 151-161.